Resumen
ABSTRACT
The evaluation of lung tissue can be done by several approaches. While structural integrity is critical for accurate diagnosis of different pathologies, scientific research uses specific tools to facilitate evaluation based on the characteristics of the study, the needs of the researcher, and available resources. Most software tools lack versatility and are perceived as black boxes, requiring extensive knowledge of the subject and software functionality to fully utilize their capabilities. Additionally, these specialized tools, often from the private sector, are costly and require specific inputs. Thus, conducting quantitative analysis of morphological features in a qualitative manner requires substantial expertise or extensive training time to ensure accurate evaluations. Therefore, the proposed software for alveolar area quantification, developed using histological images of lung tissue stained with hematoxylin-eosin, aims to provide a user-friendly interface for the end user, enabling quick and simple analyses that can be executed on devices with limited resources. The images were obtained from different groups exposed to different concentrations of lead (5, 10, 15 mg/kg) administered intraperitoneally. This approach seeks to bridge the gap between the need for precise structural analysis and the accessibility of effective tools, enhancing the efficiency and accuracy of lung tissue evaluation in research requirements.
Procesamiento digital de imágenes para la cuantificación de áreas neumónicas en pulmones crónicamente expuestos a plomo en ratas Wistar en un modelo intergeneracional
RESUMEN
La evaluación del tejido pulmonar puede abordarse de diversas maneras. Si bien la integridad estructural es fundamental para un diagnóstico preciso de diferentes patologías, la investigación científica requiere herramientas específicas que faciliten la evaluación según las características del estudio, las necesidades del investigador y los recursos disponibles. La mayoría de las herramientas de software carecen de versatilidad y son percibidas como "cajas negras", ya que exigen un conocimiento extenso tanto del tema como del funcionamiento del software para aprovechar completamente sus capacidades. Además, estas herramientas especializadas, a menudo provenientes del sector privado, son costosas y requieren insumos específicos. Por todo esto, realizar análisis cuantitativos de características morfológicas de manera cualitativa requiere una experiencia considerable o un tiempo extenso de capacitación para garantizar evaluaciones precisas. Por lo tanto, el software propuesto para la cuantificación del área alveolar, desarrollado utilizando imágenes histológicas de tejido pulmonar teñidas con hematoxilina-eosina, tiene como objetivo proporcionar una interfaz
fácil de usar para el usuario final, permitiendo análisis rápidos y simples que pueden ejecutarse en dispositivos con recursos limitados. Las imágenes fueron obtenidas de diferentes grupos expuestos a diversas concentraciones de plomo (5, 10, 15 mg/kg) administradas por vía intraperitoneal. Este enfoque busca cerrar la brecha entre la necesidad de análisis estructurales precisos y la accesibilidad a herramientas efectivas, mejorando la eficiencia y precisión de la evaluación del tejido pulmonar en los contextos de investigación.
Citas
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